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Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of WDR18 in human lung tissue with WDR18 antibody at 2.5 μg/mL.
Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

WDR18 Antibody

CATALOG NUMBER: 6817

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
WDR18 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WDR18.
The immunogen is located within the last 50 amino acids of WDR18.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WDR18 antibody can be used for detection of WDR18 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
WDR18 antibody is predicted to not cross-react with other WDR family members.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
WDR18 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WDR18 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WDR18 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WDR18
Additional Names:
WDR18 Antibody: Ipi3, R32184_1, WD repeat-containing protein 18
Protein Accession Number:
NP_077005
Protein Gi Number:
56243583
Ncbi Gene Id Number:
57418
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WDR18 Antibody: WD repeat domain 18 (WDR18) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeats are minimally conserved regions of approximately 40 amino acids that commonly form a tertiary propeller structure. WDR18 is a 432 amino acid protein that contains six WD repeats and is encoded by a gene located on human chromosome 19. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit.
Background Reference 1:
Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.
Background Reference 2:
Gao W, Xu L, Guan R, et al. Wdr18 is required for Kupffer's vesicle formation and regulation of body asymmetry in zebrafish. PLoS One 2011; 6:e23386.
Background Reference 3:
Finkbeiner E, Haindl M, and Muller S. The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex. EMBO J. 2011; 30:1067-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6817

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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