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Western blot analysis in A549 cell line lysates (35ug/lane).
WDR32 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

WDR32 Antibody

CATALOG NUMBER: 55-300

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This WDR32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-142 amino acids from the N-terminal region of human WDR32.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCAF10
Additional Names:
DDB1- and CUL4-associated factor 10, WD repeat-containing protein 32, DCAF10, WDR32
Protein Accession Number:
Q5QP82
Protein Gi Number:
74762212
Ncbi Gene Id Number:
79269
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCAF10 may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Background Reference 1:
Jin, J., et al. Mol. Cell 23(5):709-721(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-300

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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