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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in Hela cell line lysates (35ug/lane).

WDR4 Antibody

CATALOG NUMBER: 59-281

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This WDR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-173 amino acids from the Central region of human WDR4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WDR4
Additional Names:
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 {ECO:0000255, HAMAP-Rule:MF_03056}, WD repeat-containing protein 4 {ECO:0000255, HAMAP-Rule:MF_03056}, WDR4 {ECO:0000255, HAMAP-Rule:MF_03056}
Protein Accession Number:
P57081
Protein Gi Number:
20141943
Ncbi Gene Id Number:
10785
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the WD repeat proteinfamily. WD repeats are minimally conserved regions of approximately40 amino acids typically bracketed by gly-his and trp-asp (GH-WD),which may facilitate formation of heterotrimeric or multiproteincomplexes. Members of this family are involved in a variety ofcellular processes, including cell cycle progression, signaltransduction, apoptosis, and gene regulation. This gene is excludedas a candidate for a form of nonsyndromic deafness (DFNB10), but isstill a candidate for other disorders mapped to 21q22.3 as well asfor the development of Down syndrome phenotypes. Two transcriptvariants encoding the same protein have been found for this gene.
Background Reference 1:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Hu, Y.H., et al. BMC Genomics 7, 155 (2006) :
Background Reference 4:
Cartlidge, R.A., et al. EMBO J. 24(9):1696-1705(2005)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-281

List Size:
400 ul

List Price:

$495.00

Datasheet
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