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Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 μg/mL.
Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 μg/mL.
Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

WDR5 Antibody

CATALOG NUMBER: 6823

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%), Bovine: (100%)
Immunogen:
WDR5 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human WDR5.
The immunogen is located within the first 50 amino acids of WDR5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
WDR5 antibody can be used for detection of WDR5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
WDR5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WDR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WDR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WDR5
Additional Names:
WDR5 Antibody: SWD3, BIG-3, BIG3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
Protein Accession Number:
NP_060058
Protein Gi Number:
16554627
Ncbi Gene Id Number:
11091
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WDR5 Antibody: WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.
Background Reference 1:
Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.
Background Reference 2:
Gori F and Demay MB. BIG-3, a novel WD-40 repeat protein, is expressed in the developing growth plate and accelerates chondrocyte differentiation in vitro. Endocrinology 2004; 145:1050-4.
Background Reference 3:
Gori F, Friedman LG, and Demay MB. Wdr5, a WD-40 protein, regulates osteoblast differentiation during embryonic bone development. Dev. Biol. 2006; 295:498-506.
Background Reference 4:
Ang YS, Tsai SY, Lee DF, et al. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell 2011; 145:183-97.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6823

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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