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Western blot analysis of WDR92 in human kidney tissue lysate with WDR92 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of WDR92 in human testis tissue with WDR92 antibody at 10 μg/mL.
Immunofluorescence of WDR92 in human testis tissue with WDR92 antibody at 20 μg/mL.
Western blot analysis of WDR92 in human kidney tissue lysate with WDR92 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

WDR92 Antibody

CATALOG NUMBER: 5883

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
WDR92 antibody was raised against a 21 amino acid synthetic peptide near the center of human WDR92.
The immunogen is located within amino acids 140 - 190 of WDR92.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WDR92 antibody can be used for detection of WDR92 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Purification:
WDR92 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WDR92 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WDR92 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WDR92
Additional Names:
WDR92 Antibody: WD repeat-containing protein 92, WD repeat-containing protein Monad
Protein Accession Number:
NP_612467
Protein Gi Number:
24308444
Ncbi Gene Id Number:
116143
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WDR92 Antibody: WD40 repeats are a common structural module in eukaryotic proteins, and proteins containing WD40 domains have a wide range of functions, including signal transduction, cell cycle regulation, RNA splicing, and transcription. One such protein, WDR92, also known as monad, contains two WD40 repeats is widely expressed in human tissues, especially testis. Overexpression of this protein or its binding partner RNA polymerase II-associated protein 3 (RPAP3) potentiated apoptosis and caspase-3 activation induced by TNF-α and cycloheximide, suggesting that WDR92, together with RPAP3, may function as a novel modulator of apoptosis.
Background Reference 1:
Saeki M, Irie Y, Ni L, et al. Monad, a WD40 repeat protein, promotes apoptosis induced by TNF-alpha. Biochem. Biophys. Res. Commun.2006; 342:568-72.
Background Reference 2:
Itsuki Y, Saeki M, Nakahara H, et al. Molecular cloning of novel Monad binding protein containing tetratricorticopeptide repeat domains. FEBS Lett.582:2365-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5883

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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