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Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.

WEE1 Antibody

CATALOG NUMBER: 63-399

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-634 amino acids from the C-terminal region of human WEE1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WEE1
Additional Names:
Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Protein Accession Number:
P30291
Protein Gi Number:
1351419
Ncbi Gene Id Number:
7465
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WEE1 is a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Background Reference 1:
Kawasaki, H., et al., Oncogene 22(44):6839-6844 (2003).
Background Reference 2:
Hashimoto, O., et al., Mol. Carcinog. 36(4):171-182 (2003).
Background Reference 3:
Yuan, H., et al., J. Virol. 77(3):2063-2070 (2003).
Background Reference 4:
Masaki, T., et al., Hepatology 37(3):534-543 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-399

List Size:
400 ul

List Price:

$495.00

Datasheet
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