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Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody. The lane on the right is treated with the antigen-specific peptide.

WEE1 (phospho Ser642) Antibody

CATALOG NUMBER: 79-824

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p)-L-T) derived from Human WEE1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
Predicted Molecular Weight:
100 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
WEE1
Additional Names:
WEE1A, WEE1hu, kinase WEE1
Protein Accession Number:
NP_003381.1
Protein Gi Number:
4507917
Ncbi Gene Id Number:
7465
Background:
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Background Reference 1:
Watanabe N., EMBO J. 14:1878-1891(1995).
Background Reference 2:
Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).
Background Reference 3:
Igarashi M., Nature 353:80-83(1991)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-824

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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