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Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).

WFDC1 Antibody

CATALOG NUMBER: 56-449

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This WFDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-177 amino acids from the C-terminal region of human WFDC1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WFDC1
Additional Names:
WAP four-disulfide core domain protein 1, Prostate stromal protein ps20, ps20 growth inhibitor, WFDC1, PS20
Protein Accession Number:
Q9HC57
Protein Gi Number:
22096244
Ncbi Gene Id Number:
58189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the WAP-type four disulfidecore domain family. The WAP-type four-disulfide core domain, or WAPsignature motif, contains eight cysteines forming four disulfidebonds at the core of the protein, and functions as a proteaseinhibitor in many family members. The encoded protein shares 81%amino acid identity with the rat ps20 protein, which was originallyidentified as a secreted growth inhibitor. This gene is mapped tochromosome 16q24, an area of frequent loss of heterozygosity incancers, including prostate, breast and hepatocellular cancers andWilms' tumor. Owing to its location and a possible growthinhibitory property of its gene product, this gene is suggested tobe a tumor suppressor gene.
Background Reference 1:
Briggs, F.B., et al. Genes Immun. 11(3):199-208(2010)
Background Reference 2:
Madar, S., et al. Carcinogenesis 30(1):20-27(2009)
Background Reference 3:
Liu, S., et al. Clin. Exp. Metastasis 26(7):739-749(2009)
Background Reference 4:
Alvarez, R., et al. J. Virol. 82(1):471-486(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-449

List Size:
400 ul

List Price:

$495.00

Datasheet
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