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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed with 42-563 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-563 (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed with 42-563 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-563 (1mg/ml) in Lane B. Primary

WFDC2 Antibody

CATALOG NUMBER: 42-563

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: GAEKTGVCPELQADQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human WFDC2 (myc and DYKDDDDK tagged), a band of approx. 25kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
WFDC2
Additional Names:
WFDC2, WAP four-disulfide core domain 2, EDDM4, HE4, MGC57529, WAP5, dJ461P17.6, WAP domain containing protein HE4-V4, epididymal protein 4, epididymal secretory protein E4, epididymis-specific, whey-acidic protein type, four-disulfide core, major epididy
Protein Accession Number:
NP_006094.3
Protein Gi Number:
56699495
Ncbi Gene Id Number:
10406

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-563

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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