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Western blot analysis of WFDC2 in A549 cell lysate with WFDC2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of WFDC2 in human lung tissue with WFDC2 antibody at 2.5 μg/mL.
Immunofluorescence of WFDC2 in human lung tissue with WFDC2 antibody at 20 μg/mL.
Western blot analysis of WFDC2 in A549 cell lysate with WFDC2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

WFDC2 Antibody

CATALOG NUMBER: 7459

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (73%)
Immunogen:
WFDC2 antibody was raised against a 16 amino acid peptide near the amino terminus of human WFDC2.
The immunogen is located within the first 50 amino acids of WFDC2 .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WFDC2 antibody can be used for detection of WFDC2 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
WFDC2 antibody is human specific. Multiple isoforms of WFDC2 are known to exist.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 14 kDa
Observed: 20kDa
Purification:
WFDC2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WFDC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WFDC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
WFDC2
Additional Names:
WFDC2 Antibody : HE4, WAP5, EDDM4, dJ461P17.6, HE4, WAP four-disulfide core domain protein 2, Epididymal secretory protein E4
Protein Accession Number:
NP_006094
Protein Gi Number:
56699495
Ncbi Gene Id Number:
10406
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WAP four-disulfide core domain protein 2 (WFDC2), also known as epididymal protein 4, is a member of the WFDC domain family, a family of proteins that is characterized by the presence of Whey Acidic Protein (WAP) domain, and is highly expressed in the lung and salivary gland (1,2). Members of this family include SLPI and elafin, antiproteinases involved in the innate immune system (2). WFDC2 has been proposed to play a critical role in tumor formation and growth in ovarian cancer cells through the regulation of growth- and apoptosis-associated genes and may thus be a potential therapeutic target for epithelial ovarian cancer (3).
Background Reference 1:
Drapkin R, von Horsten HH, Lin Y, et al. Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serious and endometrioid ovarian carcinomas. Cancer Res. 2005; 65:2162-9.
Background Reference 2:
Bingle L, Cross SS, High AS, et al. WFDC2 (HE4): A potential role in the innate immunity of the oral cavity and respiratory tract and the development of adenocarcinomas of the lung. Resp. Res. 2006; 7:61.
Background Reference 3:
Chen Y, Mu X, Wang S, et al. WAP four-disulfide core domain protein 2 mediates the proliferation of human ovarian cancer cells through the regulation of growth- and apoptosis-associated genes. Oncol. Rep. 2013; 29:288-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7459

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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