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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

WHSC1 Antibody

CATALOG NUMBER: 58-927

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This WHSC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1089 amino acids from the C-terminal region of human WHSC1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
152 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WHSC1
Additional Names:
Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, MMSET, Nuclear SET domain-containing protein 2, NSD2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein, WHSC1, WHSC1, KIAA1090, MMSET, NSD2, TRX5
Protein Accession Number:
O96028
Ncbi Gene Id Number:
7468
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that contains four domainspresent in other developmental proteins: a PWWP domain, an HMG box,a SET domain, and a PHD-type zinc finger. It is expressedubiquitously in early development. Wolf-Hirschhorn syndrome (WHS)is a malformation syndrome associated with a hemizygous deletion ofthe distal short arm of chromosome 4. This gene maps to the 165 kbWHS critical region and has also been involved in the chromosomaltranslocation t(4;14)(p16.3;q32.3) in multiple myelomas.Alternative splicing of this gene results in multiple transcriptvariants encoding different isoforms. Some transcript variants arenonsense-mediated mRNA (NMD) decay candidates, hence notrepresented as reference sequences.
Background Reference 1:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 2:
Kang, H.B., et al. FEBS Lett. 583(12):1880-1886(2009)
Background Reference 3:
Kassambara, A., et al. Biochem. Biophys. Res. Commun. 379(4):840-845(2009)
Background Reference 4:
Brito, J.L., et al. Haematologica 94(1):78-86(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-927

List Size:
400 ul

List Price:

$469.00

Out of stock

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