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Western blot analysis of WIF1 using rabbit polyclonal WIF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (Human C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of WIF1 using rabbit polyclonal WIF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2).

WIF1 Antibody

CATALOG NUMBER: 61-453

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Rat, Xenopus
Immunogen:
This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human WIF1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WIF1
Additional Names:
Wnt inhibitory factor 1, WIF-1, WIF1
Protein Accession Number:
Q9Y5W5
Protein Gi Number:
61252765
Ncbi Gene Id Number:
11197
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Background Reference 1:
Elston,M.S., Endocrinology 149 (3), 1235-1242 (2008)
Background Reference 2:
Clement,G., Cancer Sci. 99 (1), 46-53 (2008)
Background Reference 3:
Chan,S.L., Lab. Invest. 87 (7), 644-650 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-453

List Size:
400 ul

List Price:

$495.00

Datasheet
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