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46-596 (0.03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing WIPF1 and probed with 46-596 (mock transfection in first lane).
46-596 (0.03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

WIPF1 Antibody

CATALOG NUMBER: 46-596

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KLARNESRSGSNRRE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 55kDa band observed in Human Lymph Node, Spleen and Tonsil lysates (calculated MW of 51.3kDa according to NP_003378.3 and NP_001070737.1). In transfected HEK293 transiently expressing WIPF1 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.
Specificity:
Both variants represent identical proteins
Positive Control 1:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
WIPF1
Additional Names:
WIPF1, WAS/WASL interacting protein family, member 1 , MGC111041, PRPL-2, WASPIP, WIP, Wiskott-Aldrich syndrome protein interacting protein
Protein Accession Number:
NP_003378.3,NP_001070737.1
Protein Gi Number:
38373695
Ncbi Gene Id Number:
7456
Background Reference 1:
Massarwa R, Carmon S, Shilo BZ, Schejter ED. WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila. Dev Cell. 2007 Apr;12(4):557-69.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-596

List Size:
0.1 mg

List Price:

$430.00

Out of stock

Datasheet
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