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Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 μg/mL.
Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

WIPI1 Antibody

CATALOG NUMBER: 7041

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1.
The immunogen is located within amino acids 350 - 400 of WIPI1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WIPI1 antibody can be used for detection of Wipi1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1472 - Rat Colon Tissue Lysate
Predicted Molecular Weight:
49 kDa
Purification:
WIPI1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WIPI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WIPI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WIPI1
Additional Names:
WIPI1 Antibody: ATG18, ATG18A, WIPI49, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog, WIPI-1
Protein Accession Number:
NP_060453
Protein Gi Number:
157388939
Ncbi Gene Id Number:
55062
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WIPI1 Antibody: WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.
Background Reference 1:
Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10.
Background Reference 2:
Jeffries TR, Dove SK, Michell RH, et al. PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49. Mol. Biol. Cell. 2004;15:2652-63.
Background Reference 3:
Proikas-Cezanne T, Waddell S, Gaugel A, et al. WIPI-1 (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy. Oncogene 2004; 23:9314-25.
Background Reference 4:
Proikas-Cezanne T, Ruckerbauer S, Stierhof YD, et al. Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy. FEBS Lett. 2007; 581:3396-404.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7041

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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