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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

WNK1 Antibody

CATALOG NUMBER: 61-362

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This WNK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1848-1882 amino acids from human WNK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
251 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WNK1
Additional Names:
Serine/threonine-protein kinase WNK1, 2.7.11.1, Erythrocyte 65 kDa protein, p65, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, hWNK1, WNK1, HSN2, KDP, KIAA0344, PRKWNK1
Protein Accession Number:
Q9H4A3
Protein Gi Number:
296453029
Ncbi Gene Id Number:
65125
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L.
Background Reference 1:
Verissimo F.,et al.Oncogene 20:5562-5569(2001).
Background Reference 2:
Vidal-Petiot E.,et al.PLoS ONE 7:E37751-E37751(2012).
Background Reference 3:
Scherer S.E.,et al.Nature 440:346-351(2006).
Background Reference 4:
Moore T.M.,et al.J. Biol. Chem. 275:4311-4322(2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-362

List Size:
200 ul

List Price:

$495.00

Datasheet
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