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Western blot analysis of extracts of various cell lines, using WNK3 antibody (19-741).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using WNK3 antibody (19-741) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using WNK3 antibody (19-741) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using WNK3 antibody (19-741).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

WNK3 Antibody

CATALOG NUMBER: 19-741

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of rat WNK3
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Rat hypothalamus
Positive Control 2:
Rat CNS
Positive Control 3:
Rat brain
Predicted Molecular Weight:
Observed: 40kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
WNK3
Additional Names:
RGD1563131, serine/threonine-protein kinase WNK3
Ncbi Gene Id Number:
317420
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-741

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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