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Western blot analysis in uterus tumor cell line lysates (35ug/lane).
WNK3(PRKWNK3) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in uterus tumor cell line lysates (35ug/lane).

WNK3 Antibody

CATALOG NUMBER: 62-623

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This WNK3 (PRKWNK3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1586-1616 amino acids from the C-terminal region of human WNK3 (PRKWNK3).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
198 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WNK3
Additional Names:
Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3, WNK3, KIAA1566, PRKWNK3
Protein Accession Number:
Q9BYP7
Protein Gi Number:
353526307
Ncbi Gene Id Number:
65267
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function (Holden et al., 2004 [PubMed 15194194]).[supplied by OMIM]
Background Reference 1:
Verissimo, F., et al., Oncogene 20(39):5562-5569 (2001).
Background Reference 2:
Nagase, T., et al., DNA Res. 7(4):273-281 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-623

List Size:
400 ul

List Price:

$495.00

Datasheet
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