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Western blot analysis of extracts of SKOV3 cells, using WNT2 Antibody (19-905) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using WNT2 antibody (19-905) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using WNT2 antibody (19-905) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of SKOV3 cells, using WNT2 Antibody (19-905) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

WNT2 Antibody

CATALOG NUMBER: 19-905

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-360 of human WNT2 (NP_003382.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
SKOV3
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
WNT2
Additional Names:
INT1L1, IRP, protein Wnt-2, Int-1-related protein, epididymis secretory sperm binding protein, int-1-like protein 1, secreted growth factor, wingless-type MMTV integration site family member 2
Ncbi Gene Id Number:
7472
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-905

List Size:
100 uL

List Price:

$436.00

Datasheet
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