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46-598 (1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-598 (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
46-598 (1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Detected by chemiluminescence.

WNT3 Antibody

CATALOG NUMBER: 46-598

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: CGRGHNTRTEKRKEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration: 2-4ug/ml.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
WNT3
Additional Names:
WNT3 , wingless-type MMTV integration site family, member 3 , INT4, MGC131950, MGC138321, MGC138323 , WNT-3 proto-oncogene protein precursor
Protein Accession Number:
NP_110380.1
Protein Gi Number:
13540477
Ncbi Gene Id Number:
7473
Background Reference 1:
Schmitt AM, Shi J, Wolf AM, Lu CC, King LA, Zou Y. Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping. Nature. 2006 Jan 5;439(7072):31-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-598

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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