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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
WNT3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

WNT3A Antibody

CATALOG NUMBER: 57-844

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Xenopus
Immunogen:
This WNT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human WNT3A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WNT3A
Additional Names:
Protein Wnt-3a, WNT3A
Protein Accession Number:
P56704
Protein Gi Number:
20532424
Ncbi Gene Id Number:
89780
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WNT gene family consists of structurally related geneswhich encode secreted signaling proteins. These proteins have beenimplicated in oncogenesis and in several developmental processes,including regulation of cell fate and patterning duringembryogenesis. This gene is a member of the WNT gene family. Itencodes a protein which shows 96% amino acid identity to mouseWnt3A protein, and 84% to human WNT3 protein, another WNT geneproduct. This gene is clustered with WNT14 gene, another familymember, in chromosome 1q42 region.
Background Reference 1:
Sengupta, P.K., et al. J. Biol. Chem. 285(41):31164-31173(2010)
Background Reference 2:
Hubner, R., et al. Biochem. Biophys. Res. Commun. 400(3):358-362(2010)
Background Reference 3:
von Marschall, Z., et al. Biochem. Biophys. Res. Commun. 400(3):299-304(2010)
Background Reference 4:
Shin, H., et al. J. Dermatol. Sci. 58(2):91-96(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-844

List Size:
400 ul

List Price:

$469.00

Out of stock

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