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Western blot analysis of WNT4 antibody in 293 cell line lysates (35ug/lane)
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma reacted with WNT4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using WNT4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of WNT4 antibody in 293 cell line lysates (35ug/lane)

WNT4 Antibody

CATALOG NUMBER: 62-400

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human WNT4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WNT4
Additional Names:
Protein Wnt-4, WNT4
Protein Accession Number:
P56705
Protein Gi Number:
20532425
Ncbi Gene Id Number:
54361
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WNT family consists of secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. It play a concerted role in both the control of female development and the prevention of testes formation.
Background Reference 1:
Kuulasmaa,T., Horm. Metab. Res. 40 (10), 668-673 (2008) Miyakoshi,T., Endocr. Pathol. 19 (4), 261-273 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-400

List Size:
400 ul

List Price:

$495.00

Datasheet
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