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Western blot analysis of WNT5 in Jurkat cell lysate with WNT5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of WNT5 in rat colon tissue with WNT5 antibody at 5 μg/mL.
Western blot analysis of WNT5 in Jurkat cell lysate with WNT5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

WNT5 Antibody

CATALOG NUMBER: 8063

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (93%)
Immunogen:
WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B.
The immunogen is located within amino acids 130 - 180 of WNT5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Predicted: 39 kDa
Observed: 465 kDa
Purification:
WNT5 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WNT5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WNT5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
WNT5B
Additional Names:
Wingless-type MMTV integration site family member 5
Protein Accession Number:
NP_110402
Protein Gi Number:
17402919
Ncbi Gene Id Number:
81029
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1,2). WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3). Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4).
Background Reference 1:
Saitoh T and Katoh M. Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region. Int. J. Oncol. 2001; 19:347-51.
Background Reference 2:
Serio RN. Wnt of the two horizons: putting stem cell self-renewal and cell fate determination into context. Stem Cells Dev. 2014; epub Apr. 24.
Background Reference 3:
Yang Y, Topol L, Lee H, et al. Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation. Development 2003; 130:1003-15.
Background Reference 4:
Saitoh T and Katoh M. Expression and regulation of WNT5A and WNT5B in human cancer: up-regulation of WNT5A by TNFalpha in MKN45 cells and up-regulation of WNT5B by beta-estradiol in MCF-7 cells. Int. J. Mol. Med. 2002; 10:345-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8063

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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