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Western blot analysis of extracts of mouse lung, using WNT9A antibody (23-293) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (23-293) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using WNT9A antibody (23-293) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse lung, using WNT9A antibody (23-293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

WNT9A Antibody

CATALOG NUMBER: 23-293

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse lung
Predicted Molecular Weight:
Observed: 30kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
WNT9A
Additional Names:
Protein Wnt-9a, Protein Wnt-14, WNT9A, WNT14
Ncbi Gene Id Number:
7483
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-293

List Size:
100 uL

List Price:

$436.00

Datasheet
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