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WRCH1 Antibody

CATALOG NUMBER: 46-599

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: PPQQGDPAFPDRCEA
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RHOU
Additional Names:
RHOU, ras homolog gene family, member U, ARHU, WRCH1, hG28K, WRCH-1, CDC42L1, FLJ10616, DJ646B12.2, fJ646B12.2, 2310026M05Rik, ras homolog gene family, member U, Ryu GTPase, CDC42-like GTPase, GTP-binding protein like 1, Wnt-1 responsive Cdc42 homolog, FLJ10616, WRCH1, GTP-binding protein SB128, GTP-binding protein like 1, ras-like gene family member U
Protein Accession Number:
NP_067028.1
Protein Gi Number:
11034843
Ncbi Gene Id Number:
58480
Background Reference 1:
Tao W, Pennica D, Xu L, Kalejta RF, Levine AJ. Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. Genes Dev. 2001 Jul 15;15(14):1796-807.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-599

List Size:
0.1 mg

List Price:

$391.00

Datasheet
Shipping Info

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