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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

WRN Antibody

CATALOG NUMBER: 56-216

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human WRN.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
162 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WRN
Additional Names:
Werner syndrome ATP-dependent helicase, DNA helicase, RecQ-like type 3, RecQ3, Exonuclease WRN, 31--, RecQ protein-like 2, WRN, RECQ3, RECQL2
Protein Accession Number:
Q14191
Protein Gi Number:
322510082
Ncbi Gene Id Number:
7486
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the RecQ subfamily and theDEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNAhelicases are involved in many aspects of DNA metabolism, includingtranscription, replication, recombination, and repair. This proteincontains a nuclear localization signal in the C-terminus and showsa predominant nucleolar localization. It possesses an intrinsic 3'to 5' DNA helicase activity, and is also a 3' to 5' exonuclease.Based on interactions between this protein and Ku70/80 heterodimerin DNA end processing, this protein may be involved in the repairof double strand DNA breaks. Defects in this gene are the cause ofWerner syndrome, an autosomal recessive disorder characterized bypremature aging.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Perry, J.J., et al. J. Biol. Chem. 285(33):25699-25707(2010)
Background Reference 3:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Background Reference 4:
Kobayashi, J., et al. Mech. Ageing Dev. 131(6):436-444(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-216

List Size:
400 ul

List Price:

$495.00

Datasheet
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