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46-600 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-600 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
46-600 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WRNIP1 Antibody

CATALOG NUMBER: 46-600

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: EELRGVDFFKQRRC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1kDa according to NP_064520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol Chem. 2007 Aug 10;282(32):23184-93; PMID: 17550899). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
WRNIP1
Additional Names:
WHIP, Werner helicase interacting protein, FLJ22526, putative helicase RUVBL, Werner helicase interacting protein 1, RP11-420G6.2, bA420G6.2,
Protein Accession Number:
NP_064520.2,NP_569079.1
Protein Gi Number:
18426904
Ncbi Gene Id Number:
56897
Background Reference 1:
Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T. A novel protein interacts with the Werner's syndrome gene product physicallyand functionally. J Biol Chem. 2001 Jun 8;276(23):20364-9. Epub 2001 Apr 11.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-600

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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