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Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of WWC1 in mouse brain tissue with WWC1 antibody at 5 μg/ml.
Immunofluorescence of WWC1 in mouse brain tissue with WWC1 antibody at 20 μg/ml.
Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

WWC1 Antibody

CATALOG NUMBER: 7991

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1.
The immunogen is located within amino acids 60 - 110 of WWC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 123 kDa
Observed: 110 kDa
Purification:
WWC1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WWC1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WWC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
WWC1
Additional Names:
WW and C2 domain containing 1, HBEBP3, HBEBP36, KIBRA, MEMRYQTL
Protein Accession Number:
NP_001155133
Protein Gi Number:
242247251
Ncbi Gene Id Number:
23286
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and –B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).
Background Reference 1:
Kremerskothen J, Plaas C, Buther K, et al. Characterization of KIBRA, a novel WW domain-containing protein. Biochem. Biophys. Res. Commun. 2003; 300:862-7.
Background Reference 2:
Papassotiropoulos A, Stephan DA, Huentelman MJ, et al. Common Kibra alleles are associated with human memory performance. Science 2006; 314:475-8.
Background Reference 3:
Genevet A, Wehr MC, Brain R, et al. Kibra is a regulator of the Salvador/Warts/Hippo signaling network. Dev. Cell 2010; 18:309-16.
Background Reference 4:
Xiao L, Chen Y, Ji M, et al. KIBRA protein phosphorylation is regulated by mitotic kinase aurora and protein phosphatase 1. J. Biol. Chem. 2011; 286:36304-15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7991

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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