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Western blot analysis of XAF-1 in human brain tissue lysate with XAF-1 antibody at (A) 0.5 and (B) 1 μg/mL..
Immunofluorescence of XAF-1 in Human Spleen cells with XAF-1 antibody at 20 μg/mL.
Western blot analysis of XAF-1 in human brain tissue lysate with XAF-1 antibody at (A) 0.5 and (B) 1 μg/mL..
Trial Size

XAF-1 Antibody

CATALOG NUMBER: 3409

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1.
The immunogen is located within amino acids 230 - 280 of XAF-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 - 1 μg/mL. Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN). For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 31, 33 kDa
Observed: 31 kDa
Purification:
XAF-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
XAF-1antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
XAF1
Additional Names:
XAF-1 Antibody: BIRC4BP, XIAPAF1, HSXIAPAF1, BIRC4BP, XIAP-associated factor 1, BIRC4-binding protein
Protein Accession Number:
CAA68030
Protein Gi Number:
1869901
Ncbi Gene Id Number:
54739
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
Background Reference 1:
Liston P, Fong W, Kelly NL, et al. Identification of XAF1 as an antagonist of XIAP anticaspase activity. Nature Cell Biol. 2001;3:128-33.
Background Reference 2:
Deveraux QL, Takahashi R, Savesan GS, and Reed JC. X-linked IAP is a direct inhibitor of cell-death proteases. Nature 1997;388:300-4.
Background Reference 3:
Fong WG, Liston P, Rajcan-Separovic E, et al. Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 2000;70:113-122.
Background Reference 4:
Leaman DW, Chawla-Sarkar M, Vyas K, et al. Identification of X-linked inhibitor of apoptosis associated factor-1 as an interferon-stimulated gene that augments TRAIL/Apo2L-induced apoptosis. J. Biol. Chem. 2002;277:28504-11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3409

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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