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Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.
Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 4 μg/mL.
Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.
Trial Size

XBP-1 Antibody [3H1G4]

CATALOG NUMBER: PM-4935

Clonality:
Monoclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 95-109 - XBP-1 Recombinant Protein
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Purification:
XBP-1 Monoclonal Antibody is Protein A purified.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
XBP-1 monoclonal antibody can be stored at -20°C, stable for one year.
Ncbi Official Symbol:
XBP1
Additional Names:
XBP-1 Antibody [3H1G4] : XBP2, TREB5, XBP-1
Protein Accession Number:
BAB82982
Protein Gi Number:
18148382
Ncbi Gene Id Number:
7494
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
XBP-1 Monoclonal Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Background Reference 1:
Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
Background Reference 2:
Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
Background Reference 3:
Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
Background Reference 4:
Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-4935

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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