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SDS-PAGE analysis of recombinant XBP1 on Coomassie Blue-stained 4-15% gradient gel.
SDS-PAGE analysis of recombinant XBP1 on Coomassie Blue-stained 4-15% gradient gel.

XBP1 Recombinant Protein

CATALOG NUMBER: 95-109

Tested Applications:
ELISA, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
aa 2 - 160
Fusion Tag:
Fusion Partner: N-terminal His-tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
25 kDa (Calculated)
Purity:
~95%
physical-state:
Liquid
Buffer:
1X PBS containing 0.1% SDS
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
XBP1
Additional Names:
XBP-1 Antibody : XBP2, TREB5, XBP-1
Protein Accession Number:
BAB82982
Protein Gi Number:
18148382
Ncbi Gene Id Number:
7494
Background:
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) (1). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).
Background Reference 1:
Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
Background Reference 2:
Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
Background Reference 3:
Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
Background Reference 4:
Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-109

List Size:
0.05 mg

List Price:

$289.00

Datasheet
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