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Western blot analysis of XIAP in human kidney lysate with XIAP antibody at (A) 0.5, (B) 1 , and (C) 2 μg/mL, respectively.
Immunohistochemical staining of human kidney tissue using XIAP antibody at 2 μg/mL.
Immunofluorescence of XIAP in Human Kidney tissue with XIAP antibody at 10 μg/mL.
Immunohistochemistry of XIAP in mouse kidney tissue with XIAP antibody at 5 μg/mL.
Immunofluorescence of XIAP in mouse kidney tissue with XIAP antibody at 20 μg/mL.<br><br>Red: XIAP Antibody (3331) <br> Blue: DAPI staining
Western blot analysis of XIAP in human kidney lysate with XIAP antibody at (A) 0.5, (B) 1 , and (C) 2 μg/mL, respectively.
Trial Size

XIAP Antibody

CATALOG NUMBER: 3331

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Pig: (85%), Chicken: (91%)
Immunogen:
XIAP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP.
The immunogen is located within amino acids 420 - 470 of XIAP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
XIAP antibody can be used for the detection of XIAP by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1324 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 55 kDa
Observed: 53 kDa
Purification:
XIAP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
XIAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
XIAP antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
XIAP
Additional Names:
XIAP Antibody: API3, ILP1, MIHA, XLP2, BIRC4, IAP-3, hIAP3, hIAP-3, API3, IAP3, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, ILP
Protein Accession Number:
NP_001158
Protein Gi Number:
32528299
Ncbi Gene Id Number:
331
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
XIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The X-chromosome linked inhibitor of apoptosis (XIAP) contains 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7, and -9. Upregulation of XIAP expression can protect cells from apoptosis induced by low level radiation; conversely, decreased XIAP expression by antisense targeting resulted in increased cell death following low level radiation. Two negative regulators, termed XAF-1 and Smac, can bind and inhibit XIAP activity.
Background Reference 1:
Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
Background Reference 2:
Deveraux QL, Takahashi R, Savesan GS, et al. X-linked IAP is a direct inhibitor of cell-death proteases. Nature 1997; 388:300-4.
Background Reference 3:
Deveraux QL, Leo E, Stennicke HR, et al. Cleavage of human inhibitor of apoptosis protein XIAP results in fragments with distinct specificities for caspases. EMBO J. 1999; 18:5242-51.
Background Reference 4:
Holcik M, Yeh C, Korneluk RG, et al. Translational upregulation of X-linked inhibitor of apoptosis (XIAP) increases resistance to radiation induced cell death. Oncogene 2000; 19:4174-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3331

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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