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42-013 (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-013 (0.1ug/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-013 (0.1ug/ml) staining of Pig Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-013 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-013 (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

XPNPEP1 Antibody

CATALOG NUMBER: 42-013

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow, Dog
Immunogen:
The immunogen for this antibody is: C-LIRETQPISKQH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines, and 75kDa band observed in Pig Pancreas lysate (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
XPNPEP1
Additional Names:
XPNPEP1, X-prolyl aminopeptidase (aminopeptidase P) 1, soluble, SAMP, XPNPEP, XPNPEPL, XPNPEPL1, OTTHUMP00000020457, OTTHUMP00000058856, X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (SAMP, XPNPEP, XPNPEPL), X-prolyl aminopeptidase (aminopeptidase P)-like
Protein Accession Number:
NP_065116.2,NP_001161076.1
Protein Gi Number:
21359929
Ncbi Gene Id Number:
7511
Background Reference 1:
Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nature biotechnology 2003 May 21 (5): 566-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-013

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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