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Western blot analysis of XPOT antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with XPOT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of XPOT antibody in NCI-H460 cell line lysates (35ug/lane)

XPOT Antibody

CATALOG NUMBER: 63-075

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This XPOT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 935-962 amino acids from the C-terminal region of human XPOT.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
110 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
XPOT
Additional Names:
Exportin-T, Exportin(tRNA), tRNA exportin, XPOT
Protein Accession Number:
O43592
Ncbi Gene Id Number:
11260
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
XPOT is a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN.
Background Reference 1:
Li,S.RNA Biol(4), 145-149 (2006)
Background Reference 2:
Suzuki,T., Biomed. Res. 27 (2), 89-92 (2006)
Background Reference 3:
Kuersten,S., Mol. Cell. Biol. 22 (16), 5708-5720 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-075

List Size:
400 ul

List Price:

$495.00

Datasheet
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