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Antibody used in IHC on Human Pancreas at none (Lane 2: 15ug MCF7 lysate).
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1. 30 ug MiaPaca-2 cell lysate, Lane 2. 30 ug Panc-1 cell lysate).
Antibody used in IHC on Human A549 at 1:100.
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Antibody used in IHC on Human Pancreas at none (Lane 2: 15ug MCF7 lysate).

XRCC5 Antibody

CATALOG NUMBER: 28-007

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human XRCC5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
XRCC5 antibody can be used for detection of XRCC5 by ELISA at 1:12500. XRCC5 antibody can be used for detection of XRCC5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
83 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store XRCC5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
XRCC5
Additional Names:
XRCC5, KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
Protein Accession Number:
NP_066964
Protein Gi Number:
10863945
Ncbi Gene Id Number:
7520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V (D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V (D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Background Reference 1:
Sawchuk, D.J., et al., (2004) J. Biol. Chem. 279 (28), 29821-29831.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-007

List Size:
100 ul

List Price:

$473.00

Datasheet
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