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45-173 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-173 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-173 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

XRCC5 Antibody

CATALOG NUMBER: 45-173

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SLAKKDEKTDTLED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of 75kDa was observed consistent with products from other sources.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
XRCC5
Additional Names:
XRCC5, KU80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80kDa) , HGNC:12833, KARP-1, KARP1, Ku86, NFIV , ATP-dependent DNA helicase II, DNA repair protein XRCC5, Ku86 autoantigen related protein 1, Ku autoantigen, 80kD, X-ray repair, complementing defective, repair in Chinese hamster
Protein Accession Number:
NP_066964.1
Protein Gi Number:
10863945
Ncbi Gene Id Number:
7520
Background Reference 1:
Mayeur GL, Kung WJ, Martinez A, Izumiya C, Chen DJ, Kung HJ. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem. 2005 Mar 18;280(11):10827-33. Epub 2005 Jan 7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-173

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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