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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

XYLT1 Antibody

CATALOG NUMBER: 56-641

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This XYLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human XYLT1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
108 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
XYLT1
Additional Names:
Xylosyltransferase 1, Peptide O-xylosyltransferase 1, Xylosyltransferase I, XT-I, XylT-I, XYLT1, XT1
Protein Accession Number:
Q86Y38
Protein Gi Number:
71164803
Ncbi Gene Id Number:
64131
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This locus encodes a xylosyltransferase enzyme. Theencoded protein catalyzes transfer of UDP-xylose to serine residuesof an acceptor protein substrate. This transfer reaction isnecessary for biosynthesis of glycosaminoglycan chains. Mutationsin this gene have been associated with increased severity ofpseudoxanthoma elasticum.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Muller, B., et al. J. Biol. Chem. 284(45):30775-30782(2009)
Background Reference 3:
Ponighaus, C., et al. Am. J. Hypertens. 22(4):432-436(2009)
Background Reference 4:
Ambrosius, M., et al. Clin. Biochem. 42 (1-2), 1-4 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-641

List Size:
400 ul

List Price:

$495.00

Datasheet
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