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Western blot analysis of extracts from COS7 tissue using YAP (Phospho-Ser127) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from COS7 tissue using YAP (Phospho-Ser127) Antibody. The lane on the right is treated with the antigen-specific peptide.

YAP (phospho Ser127) Antibody

CATALOG NUMBER: 80-002

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
YAP (phospho Ser127) antibody was raised against a peptide sequence around phosphorylation site of serine127 (A-H-S (p)-S-P) derived from Human YAP .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of YAP only when phosphorylated at serine 127.
Predicted Molecular Weight:
65 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
YAP1
Additional Names:
YAP65, YAP1
Protein Accession Number:
NP_001123617.1
Protein Gi Number:
194306653
Ncbi Gene Id Number:
10413
Background:
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.
Background Reference 1:
Sorrentino G, et al. (2014) Nat Cell Biol 16, 357-66
Background Reference 2:
Yang S, et al. (2014) Cell Signal 26, 343-51
Background Reference 3:
Sun W, et al. (2014) Mol Biol Rep 41, 947-56

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

80-002

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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