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Western blot analysis of extracts of HeLa cells, using YAP1 antibody (14-188).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HeLa cells, using YAP1 antibody (14-188).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

YAP1 Antibody

CATALOG NUMBER: 14-188

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Zebrafish
Immunogen:
Recombinant protein of human YAP1
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 65kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
YAP1
Additional Names:
YAP1, YAP, YAP2, YAP65, YKI
Ncbi Gene Id Number:
10413
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-188

List Size:
50 uL

List Price:

$369.00

Out of stock

Datasheet
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