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Western blot analysis of YAP1 antibody in NCI-H460 cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of YAP1 antibody in NCI-H460 cell line lysates (35ug/lane)

YAP1 Antibody

CATALOG NUMBER: 62-327

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This YAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-446 amino acids from the C-terminal region of human YAP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YAP1
Additional Names:
Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1, YAP65
Protein Accession Number:
P46937
Protein Gi Number:
294862479
Ncbi Gene Id Number:
10413
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YAP1 is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
Background Reference 1:
Cao,X., Genes Dev. 22 (23), 3320-3334 (2008)
Background Reference 2:
Yokoyama,T., Carcinogenesis 29 (11), 2139-2146 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-327

List Size:
400 ul

List Price:

$495.00

Datasheet
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