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Western blot analysis of extracts of various cell lines, using YES1 antibody (13-225) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using YES1 antibody (13-225) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using YES1 antibody (13-225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

YES1 Antibody

CATALOG NUMBER: 13-225

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human YES1 (NP_005424.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
HepG2
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse heart
Predicted Molecular Weight:
Observed: 61kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
YES1
Additional Names:
YES1, C-yes, Cyes, Cellular yes-1 protein, HsT441, Oncogene kinase yes1, p61-YES, p61yes, p62c-yes, Proto-oncogene c-Yes, Tyrosine-protein kinase Yes, Yes kinase, v-yes, Yes, Yamaguchi sarcoma oncogene
Ncbi Gene Id Number:
7525
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-225

List Size:
100 uL

List Price:

$436.00

Datasheet
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