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Western blot analysis of YMEL1 Antibody in HepG2 cell line lysates (35ug/lane)
Western blot analysis of YMEL1 Antibody in CHO cell line lysates (35ug/lane)
YMEL1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of YMEL1 Antibody in HepG2 cell line lysates (35ug/lane)

YMEL1 Antibody

CATALOG NUMBER: 61-697

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This YMEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-219 amino acids from the N-terminal region of human YMEL1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YME1L1
Additional Names:
ATP-dependent zinc metalloprotease YME1L1, 3424-, ATP-dependent metalloprotease FtsH1, Meg-4, Presenilin-associated metalloprotease, PAMP, YME1-like protein 1, YME1L1, FTSH1, YME1L
Protein Accession Number:
Q96TA2
Protein Gi Number:
46397258
Ncbi Gene Id Number:
10730
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YMEL1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.
Background Reference 1:
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Background Reference 2:
Deloukas, P., et al. Nature 429(6990):375-381(2004)
Background Reference 3:
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-697

List Size:
400 ul

List Price:

$495.00

Datasheet
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