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Western blot analysis of YPEL1 in Hela cell lysate with YPEL1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of YPEL1 in HeLa cells with YPEL1 antibody at 2.5 μg/mL.
Immunofluorescence of YPEL1 in HeLa cells with YPEL1 antibody at 20 μg/mL.
Western blot analysis of YPEL1 in Hela cell lysate with YPEL1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

YPEL1 Antibody

CATALOG NUMBER: 6771

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
YPEL1 antibody was raised against a 11 amino acid synthetic peptide near the amino terminus of human YPEL1.
The immunogen is located within the first 50 amino acids of YPEL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
YPEL1 antibody can be used for detection of YPEL1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
YPEL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
YPEL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
YPEL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YPEL1
Additional Names:
YPEL1 Antibody: FKSG3, Protein yippee-like 1
Protein Accession Number:
NP_037445
Protein Gi Number:
9558721
Ncbi Gene Id Number:
29799
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YPEL1 Antibody: YPEL1 (yippee-like 1) is thought to play a role in the epitheloid conversion of fibroblasts and the regulation of the cellular morphology. It is a 119 amino acid protein that localizes to the nucleus and belongs to a family of yippee-like proteins that include YPEL2 - 5, all of which localize to the centrosome and nucleus and are widely expressed in both adult and fetal tissue. The family of human YPEL proteins share a high degree of sequence homology with their rodent homologs, suggesting a conserved function between species. YPEL1 might be an important factor during the development and malignant transformation of tissues.
Background Reference 1:
Farlie P, Reid C, Wilcox S, et al. YPEL1: a novel nuclear protein that induces an epithelial-like morphology in fibroblasts. Genes Cells 2001; 6:619-29.
Background Reference 2:
Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43
Background Reference 3:
Abiatari I, Kiladze M, Kerkadze V, et al. Expression of YPEL1 in pancreatic cancer cell lines and tissues. Georgian Med. News 2009; (175):60-2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6771

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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