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Western blot analysis of lysate from Hela cell line, using YWHAG Antibody at 1:1000 at each lane.
Western blot analysis of YWHAG Antibody in mouse cerebellum tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with YWHAG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of widr cells using YWHAG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of YWHAG using rabbit polyclonal YWHAG Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAG gene.
Western blot analysis of lysate from Hela cell line, using YWHAG Antibody at 1:1000 at each lane.

YWHAG Antibody

CATALOG NUMBER: 61-614

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat, Xenopus
Immunogen:
This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human YWHAG.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YWHAG
Additional Names:
14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma, N-terminally processed, YWHAG
Protein Accession Number:
P61981
Protein Gi Number:
48428721
Ncbi Gene Id Number:
7532
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Background Reference 1:
Jagemann,L.R., et.al., J. Biol. Chem. 283 (25), 17450-17462 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-614

List Size:
400 ul

List Price:

$495.00

Datasheet
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