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Western blot analysis of YWHAZ Antibody in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane)
YWHAZ Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of YWHAZ Antibody in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane)

YWHAZ Antibody

CATALOG NUMBER: 63-430

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat
Immunogen:
This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the Central region of human YWHAZ.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YWHAZ
Additional Names:
14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Protein Accession Number:
P63104
Protein Gi Number:
52000887
Ncbi Gene Id Number:
7534
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Background Reference 1:
Powell, D.W., et al., Mol. Cell. Biol. 23(15):5376-5387 (2003).
Background Reference 2:
Zhu, P., et al., Biochem. Biophys. Res. Commun. 301(4):991-999 (2003).
Background Reference 3:
Li, Y., et al., J. Biol. Chem. 277(47):44593-44596 (2002).
Background Reference 4:
Wang, H., et al., J. Clin. Endocrinol. Metab. 87(6):2629-2634 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-430

List Size:
400 ul

List Price:

$495.00

Datasheet
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