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Western blot analysis of anti-14-3-3 protein zeta/delta Anbtibody (T232) in A375 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with YWHAZ Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-14-3-3 protein zeta/delta Anbtibody (T232) in A375 cell line lysates (35ug/lane)

YWHAZ Antibody

CATALOG NUMBER: 63-431

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from human YWHAZ.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YWHAZ
Additional Names:
14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Protein Accession Number:
P63104
Protein Gi Number:
52000887
Ncbi Gene Id Number:
7534
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
14-3-3 protein zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs.
Background Reference 1:
Li,F.Q., J. Cell Biol. 181 (7), 1141-1154 (2008)
Background Reference 2:
Mateo,I., Eur. J. Neurol. 15 (3), 219-222 (2008)
Background Reference 3:
Li,Z., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 162-167 (2008)
Background Reference 4:
Powell,D.W., Mol. Cell. Biol. 23 (15), 5376-5387 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-431

List Size:
400 ul

List Price:

$495.00

Datasheet
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