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Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293,HL-60,SiHa,K562,THP-1,U266 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.

YY1 Antibody

CATALOG NUMBER: 61-408

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
This YY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-104 amino acids from the N-terminal region of human YY1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YY1
Additional Names:
Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1, YY1, INO80S
Protein Accession Number:
P25490
Protein Gi Number:
3915889
Ncbi Gene Id Number:
7528
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Background Reference 1:
Sucharov, C.C., et al., J. Biol. Chem. 278(33):31233-31239 (2003). Kurisaki, K., et al., Mol. Cell. Biol. 23(13):4494-4510 (2003). Huang, N.E., et al., Biochem. Biophys. Res. Commun. 306(1):267-275 (2003). Moriuchi, M., et al., J. Biol. Chem. 278(15):13003-13007 (2003). Hiromura, M., et al., J. Biol. Chem. 278(16):14046-14052 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-408

List Size:
400 ul

List Price:

$519.00

Datasheet
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