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Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.
Immunohistochemical analysis of paraffin - embedded human lymph showing cytoplasmic localization using ZAP70 antibody with DAB staining.
Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.

ZAP70 Antibody [3D2A4]

CATALOG NUMBER: 32-237

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant ZAP70 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
ZAP70 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZAP70
Additional Names:
SRK, 70 kDa zeta-chain associated proteinSRK, STD, TZK, STCD, ZAP-70
Protein Accession Number:
P43403
Protein Gi Number:
1177044
Ncbi Gene Id Number:
7535
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZAP70(zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
Background Reference 1:
Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32.
Background Reference 2:
Joaquim Carreras , Neus Villamor ,Lluis Colomo J Pathol. 2005 Mar;205(4):507-13.
Background Reference 3:
Claire Hivroz Med Sci (Paris). 2005 Feb;21(2):150-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-237

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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