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42-992 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-992 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ZAP70 Antibody

CATALOG NUMBER: 42-992

Clonality:
Polyclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SDGYTPEPARITSPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificity:
This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Approx 70 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ZAP70
Additional Names:
ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD
Protein Accession Number:
NP_001070.2
Protein Gi Number:
31455611
Ncbi Gene Id Number:
7535

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-992

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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