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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
ZAP70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

ZAP70 Antibody

CATALOG NUMBER: 57-508

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human ZAP70.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
70 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZAP70
Additional Names:
Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK
Protein Accession Number:
P43403
Protein Gi Number:
1177044
Ncbi Gene Id Number:
7535
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an enzyme belonging to the proteintyrosine kinase family, and it plays a role in T-cell developmentand lymphocyte activation. This enzyme, which is phosphorylated ontyrosine residues upon T-cell antigen receptor (TCR) stimulation,functions in the initial step of TCR-mediated signal transductionin combination with the Src family kinases, Lck and Fyn. Thisenzyme is also essential for thymocyte development. Mutations inthis gene cause selective T-cell defect, a severe combinedimmunodeficiency disease characterized by a selective absence ofCD8-positive T-cells. Two transcript variants that encode differentisoforms have been found for this gene.
Background Reference 1:
Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010)
Background Reference 2:
Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) :
Background Reference 3:
Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010)
Background Reference 4:
Kotaskova, J., et al.Mol Diagn 12(3):328-334(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-508

List Size:
400 ul

List Price:

$495.00

Datasheet
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