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Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.
Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.
Trial Size

ZBED1 Antibody

CATALOG NUMBER: 5111

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ZBED1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1.
The immunogen is located within amino acids 180 - 230 of ZBED1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
ZBED1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZBED1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZBED1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBED1
Additional Names:
ZBED1 Antibody: ALTE, DREF, TRAMP, hDREF, ALTE, KIAA0785, Zinc finger BED domain-containing protein 1, Putative Ac-like transposable element
Protein Accession Number:
AAH15030
Protein Gi Number:
15929151
Ncbi Gene Id Number:
9189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
Background Reference 1:
Ohshima N, Takahashi M, and Hirose F. Identification of a human homolog of the DREF transcription factor with a potential role in regulation of the histone H1 gene. J. Biol. Chem.2003; 278:22928-38.
Background Reference 2:
Yamashita D, Sano Y, Adachi Y, et al. hDREF regulates cell proliferation and expression of ribosomal protein genes. Mol. Cell. Biol.2007; 27:2003-13.
Background Reference 3:
Yamashita D, Komori H, Higuchi Y, et al. Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding. J. Biol. Chem.2007; 282:7563-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5111

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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